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February 2013

Tetraploid grasses into Genome Wide Selection

In 2010 DLF-TRIFOLIUM launched a major research program together with Aarhus University (AU) aiming at implementing Genomw Wide Selection (GWS) in forage ryegrass breeding. Now this collaboration is about to expand into a new Centre for Genomic Selection in Animals and Plants (GenSAP) including new plant species, new breeding companies and new international expert groups, all working on the same platform to develop solutions for tomorrows genomic breeding programs. For DLF-TRIFOLIUM this initiative will pave the way for using GWS both in diploid and tetraploid ryegrass breeding.

 

GenSAP is a Danish initiative gathering grasses, wheat, barley, and potato with cattle, pig chicken and mink into one cutting edge research platform. The goal is to advance GWS into breeding of genetically more complex species, such as tetraploid grasses, and to make the system less prone to environmental effects. This development has a special interest to DLF-TRIFOLIUM, who spends a large fraction of its breeding activities on serving a still expanding market for tetraploid forage ryegrass. Fortunately, the innovations made on GWS diploid ryegrass will serve as a perfect blueprint for reaching this goal.

 

GenSAP runs over five years and has a total budget of 9.1 M €. It has received a 4.1 M € grant from the Danish Ministry of Research and Innovation.

 

September 2012

Extra production from marginal soils?

With increasing demands for feed, food, and fiber, the agricultural production system faces a future where marginal soils will be used for low-input farming to a much larger extend than what is evident today. The conditions at such areas are generally very stressful comprising exposure to extremes in pH, salinity, drought as well as nutrient efficiency. It is expected that perennial grasses are among the most well suited plants for these production conditions. The question is which species will do the job most efficiently, both in terms of biomass yield, stress tolerance, and convenience for biomass utilization and transportation.  

This big question is currently being addressed in a major EU project called GrassMargins, in which DLF together with 11 other partners from Academia and Industry, will examine and develop new varieties of Tall Fescue, Festulolium, Cocksfoot, Reed Canary Grass, and Elephant Grass (Miscanthus) for marginal soils.  

At first these five species will be tested for tolerance to various types of stress, including salinity, drought, flooding, and cold by different partners. Salinity test will be conducted by DLF-TRIFOLIUM who has developed a great expertise in screening large populations for salt tolerance using soil-free media.

 

 

Data from all tests conducted in the consortium will guide the future breeding process in making the most robust varieties for growth on marginal soils. So better start now looking in your back yard for exterior land to spike your future agricultural production – perhaps with tough grasses from DLF-TRIFOLIUM.  

 

June 2012

Development of grasses with efficient nitrogen usage

Nitrogen (N) is one of the most vital nutrient sources for plant growth setting the threshold for production levels in many situations. In many countries, however, the usage of nitrogen for plant production or for amenity is subject to environmental regulation. In addition, many plant producers, green keepers, and grounds men experience a hitherto unseen increase in fertilizer price, which is directly linked to increasing oil prices. While these constraints are only expected to increase in the future at DLF-TRIFOLIUM we are moving up better nitrogen use efficiency (NUE) as one of our top priority traits.  

In forage grasses we already started three years ago subjecting the first forage varieties to NUE tests in our computer controlled hydroponic system. This is done again this year where 40 forage perennial ryegrass varieties will be tested alongside 40 turf varieties. While the main goal for forages is to maintain dry matter yield under suboptimal N supply the main goal for turfs is to keep a good color and a high shoot density in order to prevent weed introgression. 

For future breeding we have advanced the system to include more than 1000 forage perennial ryegrass breeding lines. These lines constitute our genome wide selection program, in which each line will also be characterized with more than 100,000 genetic markers. Using massive computer power this information will allow us to select plants based on their genetic potential for NUE and also many other important traits simultaneously.

  

We do this to ensure that you will get the best choice for low input conditions – on the short term with the good varieties that are already at hand – on the long term with varieties that will surely prove to be superior to these.

 

February 2012 

ForageSelect – Home for a great new project

Genome Wide Selection (GWS) is a new and powerful selection method, which bases itself on the plants genetic potential instead of their usual trial data. It has already been developed for and revolutionized animal breeding. Now the turn has come to grasses.

To get started, a computer model needs to be developed, which can associate trial data from a large number of breeding lines with their DNA profile. Dissection of the genome into millions of data points, which contributes individually to any trait allows for selection of plants with the highest breeding values for several traits simultaneously. The ability to select for more than one trait at a time is indeed one of the great forces of GWS. In the long term GWS may also reduce the number of expensive field trials because selections are made on calculated breeding values derived from genomic data. The goal is to achieve major leaps in yield and seed yield while maintaining stress tolerance, disease resistance, and decreasing nitrogen loss and greenhouse gas emission.

 

www.forageselect.com/ is established under a Danish grant (GUDP), which supports the GWS project financially. Please visit the site to discover more on future grass inventions.

 

January 2012

Appointment: New Breeder in the Danish R&D Team

Entering 2012, it is a great pleasure for DLF-TRIFOLIUM to present a new grass breeder, Anders Søndergaard Larsen who will enter the team at Danish Plant Breeding, Store Heddinge.

Anders is 34 years old and has a Master of Science in management of forestry and natural resources from the Royal Veterinary and Agricultural University in Copenhagen. He also holds a Ph.D. degree in plant genetics at Copenhagen University, Faculty of Life sciences. During the last year he has been working on the potential of grasses for bioenergy production as post-doc at Aarhus University, Department of Genetics and Biotechnology. The employment of Anders is part of a generational change at the breeding station in Store Heddinge as our turf grass breeder Niels Christian Nielsen is planning to end his long career in the second half of 2012.

 

 

December 2011

Genomic Salt Selection

While fields are lying dark and faded in the winter cold the grass is still growing green inside the greenhouses at DLF-TRIFOLIUM's Research Division in Store Heddinge. In this environment a large experiment is set up in which more than 1000 forage ryegrass families are being tested for salt tolerance. The salt stress experiment is just one out of many trials that will deliver research data to a major research project on Genome Wide Selection.

Shortly, genome wide selection consists in selecting plants on the basis of their genetic potential rather than the usual field trial data. In order to do so, an association needs to be established between the plants DNA-profiles and the traits you wish to breed for. Aside from a large grass population this task also requires substantial expertise and massive computer power. Therefore DLF-TRIFOLIUM has joined forces with the Institute for Genetics and Biotechnology at Aarhus University, which developed the system for cattle breeding with the aim of implementing the technology into forage grass breeding.  

In this collaboration DLF-TRIFOLIUM is to test the 1000 selected ryegrass families for a number of different traits including salt tolerance. There are two major reasons why salt tolerance is given this attention. Firstly, at several places in Southern Europe and USA there are problems with soils being frequently irrigated with recycled water. This practice slowly builds up growth inhibitive depositions of salt. Secondly, there seems to be a close correlation between salt- and drought tolerance and plants showing high salt tolerance may therefore also show tolerance to drought.

Once an association between the plant's DNA profiles and stress tolerance has been calculated, the same computer can calculate the genetic stress tolerance potential for new plants entering future breeding on the basis of a DNA test. This increases predictability in the selection process and allows for improvement of not only stress tolerance but also other traits without compromising yield gains. The project is running in four years and receives financial support from Natl. programs, GUDP and The Law of Innovation.

 

December 2011

In prep for future greens premium varieties

If you want to have a glimpse of what will become the future’s most exquisite greens varieties you might want to drop by DLF TRIFOLIUM’s Research Division in Denmark and have a look at their newly established greens. 

Perhaps you have already enjoyed playing on super varieties, such as GREENSLEAVES and CEZANNE. If so, we are pleased to inform you that their upgrades are already in the pipeline. Having gone through series of selections and tests the new elite material is now ready for real-life greens management tests. A typical management scheme includes regular cutting down to 5.5 mm and fertilization with 100-120 kg N/ha/year. Such cutting heights are extremely stressful for grass plants, but with the high density and fine leaves that GREENSLEAVES and CEZANNE are famous for, it is just the right environment to highlight these grasses’ outstanding greens characteristics.  

But first the new elites will need to prove their value through the coming winter. Winter hardiness and snow mold tolerance are standard assets in today’s top varieties and our breeder, Niels Christian Nielsen, expects to see nothing else but eminent winter performance in the new material. Knowing that he is seldom proven wrong, we therefore take the liberty already now to welcome you to visit our breeding station next summer and to check out for yourself how we define premium variety material. See you!  

 

September 2011

Low Input Grasses as Recurrent Biomass Sources

Perennial grasses are not only good for amenity and for feeding they are also good for CO2 neutral energy production. And if you ask a number of scientific experts now gearing up for research in a new EU project, such grasses may also have a very sustainable input/output ratio when it comes to growing demands. The project is called "GrassMargins" and as the name indicates it aims at finding optimal grasses and optimal conditions for grass biomass production on marginal soils. The project consortium consists of 12 partners from eight countries representing Northern, Central and Western Europe, Russia and China.

Eight Academic Institutions and four companies, including DLF-TRIFOLIUM are going to investigate the properties of three C3 grasses, Tall fescue, Cocksfoot, Reed canary grass and one C4 grass, Miscanthus, in relation to low input. While Miscanthus is well known for its high biomass yield it remains to be elucidated, which of the four grasses has the overall best performance, productivity, and economy when it comes to growth under marginal and perhaps stressful conditions.

The projects will also perform pre-breeding within these species in order to improve both productivity under stressful conditions and drying after cutting in the field. DLF-TRIFOLIUM has great expertise in drought and salt tolerance and will test included breeding material in its semi-hydroponic system and in salt-affected soils in France. All material is subject to extensive modeling using the experimental data to get a qualified image of the most productive species/varieties under various marginal conditions. The consortium has a total budget of 4 million Euro and will run until October 2015.

 

August 2011

Fine fescues in the combat against Poa annua

Having trouble defeating Poa annua on your golf green? Simply turn up the slender fine fescue content and start winning your combats. If you are struggling to keep your green free of Poa annua, you may want to call for aid from some of the fine fescues that you already know.

This is one of the clear messages to be extracted from a recently finalised competition trial carried out at DLF-TRIFOLIUM’s R&D greens facilities. The initial idea was to investigate how different nitrogen levels affect the combat between sown grasses and Poa annua. In order to do this, DLF-TRIFOLIUM established a four year trial in collaboration with The Danish Golf Union and The Agricultural University (KULife). Two additional factors were also examined; wear applications and sown grass species. Poa annua taken with a hole cutter was inserted as cores into established plots of one square meter. 

In the first trial, two fine fescue mixtures were tested alongside pure varieties of the three typical mix components, Browntop, Chewings and Slender Red Fescue. While Poa annua increased in area alongside the Browntop variety, its growth was reduced 20-35% in fine fescue plots, with slender fescue being the most potent inhibitor (graph below).

In the second trial, some of the newest chewings and slender varieties were tested against each other at fixed nitrogen levels. All the slender type fescues showed the same remarkable efficiency in restricting Poa annua growth (graph below). Chewings also defeated Poa annua growth albeit at a more reduced level.

In the second trial, some of the newest chewings and slender varieties were tested against each other at fixed nitrogen levels. All the slender type fescues showed the same remarkable efficiency in restricting Poa annua growth (graph below). Chewings also defeated Poa annua growth albeit at a more reduced level.

These interesting findings will surely find their way into new golf green mixtures with higher content of slender type varieties (up to 75%) to help combat your future Poa annua problems.

 

March 2011

Novelties in France - Nine extraordinary Quality Turf varieties from DLF-TRIFOLIUM

Two new tall fescues and seven new perennial ryegrass varieties has just been top listed in the French Official Turf List.

The two tall fescues, ESSENTIAL and EYECANDY, carry American genes for dark color, with Eyecandy more pronounced than Essential. Both varieties show strong vitality, high density, and very good quality over the year.  On top of this ESSENTIAL also shows excellent wear tolerance, qualifying it directly to the top of the French list.

The seven perennial ryegrass varieties are Aiken, IS-PR 274, Clementine, Duparc, Enesco, Greenblush and Leclair.  These varieties cover the color groups from medium green (DUPARC) to the vary dark (IS-PR 274). Summer performance is very good for AIKEN, DUPARC and GREENBLUSH, but the absolute winner is CLEMENTINE.  This eye-catching variety, which at all times have been standing out from the others in the lawn trials have demonstrated extraordinary lawn qualities with clear green color, very fine leaves, excellent density, very high persistence, and tolerance to wear. Most of these very promising ryegrass varieties are also under National testing in other European countries, mainly DE, UK and NL, confirming the high qualities shown in the preliminary results.

 

February 2011

Grasses for Extreme Challenges

These years we experience an increasing demand for high quality grass varieties, which are drought or salt tolerant. Such varieties are most-wanted by golf and sport facilities placed in dry or coastal areas, where evaporation is high or where frequent penetration of seawater into the waterbed creates problems with saline irrigation especially during summer. In addition, in northern areas, de-icing roads has resulted in high salt levels along roadsides. That is why DLF-TRIFOLIUMs R&D Department for several years now has investigated the ability of grass to withstand different types of stress. The first hardy varieties have already been identified, and soon the company can deliver the first turf mixtures with salt tolerant varieties.

With climate models predicting more extreme weather in the future plant producers, green keepers, managers of recreational areas, as well as private households will be left with no other option than to use recycled water or to skip irrigation for longer periods. However, irrigation with recycled water often causes a slow and detrimental accumulation of salts in the soil. Leaf tip burns, discoloration and difficulties in controlling pests are common symptoms and implications for turf grasses stressed by elevated salt levels.

Some grass species are very sensitive to salt stress and display severe symptoms even at moderate concentrations while others, such as Tall fescue, can grow in soils with salt levels similar to that of brackish water. In order to identify the most salt tolerant varieties within DLF-TRIFOLIUMs portfolio new breeding material is continuously being tested under controlled greenhouse conditions. Well-established plants growing in soil-free media, where all nutrients and salts are precisely dosed, are subject to increasing amounts of salt. When symptoms are evident and varied within each species all varieties are evaluated and only the best selected for further testing. Such varieties are now being screened for salt tolerance under field conditions in Australia. They are also subject to drought trials at DLF-TRIFOLIMs breeding station in Oregon as these two types of stress tolerances seems to be connected.

During the last few years municipalities and regulatory authorities have also increased their focus on stress tolerance and sustainability. In extension of this awareness The National Turfgrass Evaluation Program (NTEP) in USA has now incorporated salt tolerance test as one of the compulsory elements in the extensive evaluation list. DLF-TRIFOLIUM submits every year a number putative varieties to this test. The goal is not only having single salt tolerant varieties recommended but also to deliver complete mixtures where the included species have a strong and balanced salt tolerance.

 

January 2011

Green is green and turf is turf - or is it?

Not if you ask Steve Reid, who is the charge of DLF-TRIFOLIUMs American turf breeding program. To him and many other people who are enthusiastic about their turf, turf is about esthetically good looking grasses. It’s about color, it’s about ground coverage, it’s about leaf densities, and for sport and leisure purposes it’s also about wear tolerance. So this is what Steve is aiming at, when selecting for the coming top varieties: A top quality turf grass is fine-leafed and dense, it has high tiller production to ensure good ground coverage even at very low-mowing. While preferences for specific colors differ between areas (dark green in North America, and normal green for Europe), it is important that all turf varieties maintain their good looking color over the entire growth season and be as little affected by fungal attack (rust) or drought stress.

However, among thousands of turf breeding plots, the assessment of these parameters by the human eye is easily misjudged either by changing light and weather conditions or by other types of false interpretation. To battle this situation and to achieve the most objective measurements Steve and his team employ a digital image system developed by Dr. Karcher, and Richardson of the University of Arkansas. It can take a snapshot a turf plot independently of environmental light conditions and transform the pixels into quality measurements. Covered by a square metal box the captured turf plot is only illuminated by a built-in light source. This allows for a direct comparison between plots by the image analysis software, which calculates turf color-, coverage-, and density scores based on a pre-defined scale of hue and saturation. Images taken before and after wear treatments are used to calculate wear tolerance of each breeding line. According to Steve, the system is both robust and reliable, and he has already five new varieties selected by this system in the pipeline for official testing. He wouldn’t be surprise to see similar imaging systems becoming the standard method of assessing turf quality at the National Test Centers in the future. So look out for upcoming top quality varieties. They will be more green than your eye can ever imagine.

SMART FROM THE START

During the ISF World Seed Congress 2012 in Rio de Janeiro, ISF released a video which highlights the need for new plant varieties in light of the challenges of this planet.

View the video on YouTube: